What are the Applications of Hydroxy Methionine Manganese in Animal Farming

1) Layers
In layer diets, replacing inorganic manganese and zinc with hydroxy methionine chelated manganese and zinc maintained production performance and egg quality while reducing trace mineral excretion, demonstrating efficient and eco-friendly characteristics. During the late laying period, it also tended to improve laying rate, daily egg output, and feed-to-egg ratio.

Effects of dietary supplementation of different forms of trace elements on feces and excretion of trace elements in laying hens

Note: 1: 80 mg/kg ZnSO₄, 60 mg/kg _MnSO₄; 2: 20 mg/kg ZnSO₄, 15 mg/kg _MnSO₄; 20 mg/kg _Zn-MHA, 15 mg/kg _Mn-MHA; 3: 40 mg/kg Zn-MHA, 30 mg/kg Mn-MHA. Different letters within the same color indicate significant differences among treatment groups (P < 0.05).

2) Growing–finishing pigs
In growing–finishing pigs, partial replacement (1/5–2/5) of inorganic trace minerals with Hydroxy Methionine chelated Manganese (MHA-M) not only improved average daily gain and enhanced immune and antioxidant functions but also significantly reduced fecal excretion of Cu, Fe, Mn, and Zn.

Table 1 Effect of methionine hydroxy analog chelated microminerals on immune function in growing–finishing pigs

Item	ITM 1/5	MHA-M 2/5	MHA-M 3/5	MHA-M 4/5	MHA-M	MHA-M	SEM	P value
Serum g/L
Day 35
IgA	1.03c	1.28ab	1.19b	0.80d	0.98c	1.40 a	0.03	<0.001
IgG	8.56c	8.96ab	8.94ab	8.06d	8.41 cd	9.27 a	0.07	<0.001
IgM	0.84c	0.92b	0.91b	0.75d	0.81 cd	1.00 a	0.01	<0.001
Day 70
IgA	1.28ab	1.27ab	1.35 a	1.35 a	1.12b	0.86c	0.03	<0.001
IgG	8.98ab	9.14 a	8.97ab	8.94ab	8.42bc	8.15c	0.08	<0.001
IgM	0.94 a	0.91ab	0.95 a	0.95 a	0.86b	0.78c	0.01	<0.001
Day 91
IgA	1.13ab	1.16ab	1.14ab	1.24 a	1.01b	1.03b	0.02	0.012
IgG	9.32ab	9.25ab	9.25ab	9.48 a	8.81ab	8.74b	0.08	0.014
IgM	0.88ab	0.90ab	0.90ab	0.93 a	0.83b	0.84b	0.01	0.013

Note：Within a row, different superscripts mean significant difference (P < 0.05).

ITM, a basal diet with Cu, Fe, Mn, and Zn from sulfates providing 20, 100, 40, and 60 mg/kg; MHA-M, methionine hydroxy analog chelated microminerals; SEM, standard error of mean.

3) Aquatic animals
Supplementing 30.69–45.09 mg/kg hydroxy methionine chelated manganese in the diet of Litopenaeus vannamei (Pacific white shrimp) significantly improved growth performance, antioxidant capacity, and immune function, and enhanced manganese deposition in tissues. The optimal level was 30.69 mg/kg, which increased antioxidant enzyme activity, improved lipid metabolism, and downregulated genes related to endoplasmic reticulum stress and apoptosis.

Note: Effects of different dietary Hydroxy Methionine chelated Manganese (MHA-M) levels on Mn metabolism, antioxidant nonspecific immunity, endoplasmic reticulum stress, apoptosis and lipid metabolism in L. vannamei. Red arrows represent an increase, the blue arrow indicates descent.